A regulation game consists of four 12 -minute quarters.

The game shall start promptly at the time prescribed by the AUDL. If a team is late appearing on the field for the 1st or 3rd quarter, they will be fined at the discretion of the AUDL.

The 3rd quarter pull occurs after a 10 to 15-minute halftime, determined by the home team 24 hours in advance. Any halftime over 15 minutes must be approved at least one week in advance by the Office of the Commissioner. The 2nd quarter pull, 4th quarter pull, and any overtime pulls occur 2 minutes and 30 seconds after the conclusion of the previous period of play.

The receiving team is permitted 30 seconds after a goal to signal their readiness. The pulling team is permitted 50 seconds after a goal to pull but may pull as soon as the receiving team signals readiness. Any violation is a delay of game penalty.

A team timeout lasts 90 seconds, and play must commence at the end of the 90 seconds. If the defense is not ready, the offense may resume play. If the offense is not ready, the stall count will commence if a marker is within 3 yards of where the disc will come into play.

A team is permitted 60 seconds to stop the bleeding of a player, or that player must be substituted.

A team is permitted 30 seconds to replace an ejected player.